5/16/2023 0 Comments Halo tag snapgenePaeR7I does not recognize the sequence CTCTCGAG. PshAI quickly loses activity at 37☌, but can be used at 25☌ for long incubations.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence.īssHII is typically used at 50☌, but is 75% active at 37☌. Sticky ends from different PasI sites may not be compatible. This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. Sticky ends from different BstEII sites may not be compatible. Sticky ends from different PflMI sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Ĭ C A N N N N N T G G G G T N N N N N A C C Sticky ends from different XcmI sites may not be compatible.īsrGI is typically used at 37☌, but is even more active at 60☌.
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